APPLICATION OF ELECTRON MICROSCOPY IN THE DIAGNOSIS OF KIDNEY DISEASE

Cilj: prikazati ulogu EM u rutinskoj dijagnostici bubrežnih biopsija te složenost i značaj predanalitičkog postupka Metode: opis uređaja, reagensa i predanalitičkog postupnika (kvalitetno uzorkovanje i obrada materijala, rezanje ultratankih preparata i kontrastiranje) Rezultati: izrada preparata za elektronsku mikroskopiju na Medicinskom fakultetu u Splitu Zaključci: U rutinskoj dijagnostici primarnih i sekundarnih glomerulopatija EM sa svjetlosnom mikroskopijom i imunoflorescencijom zajedno predstavlja zlatni standard za postavljanje patološke dijagnoze. U dijagnostici bubrežnih bolesti splitski patolozi će još neko vrijeme za potvrdu dijagnoze ili konzultaciju koristiti specijalizirani laboratorij i EM druge ustanove.Aim: To show the role of EM in routine diagnosis of percutanous renal biopsies and the complexity and importance of preanalytic process. Methods: A description of machines, reagents, preanaylitic algorithm and problems (quality sampling and processing of tissue, cutting ultrathin slides and contrasting). Results: ultrathin contrasted slides of renal percutaneus biopsies made in the Clinical Hospital Center and the Medical School in Split. Conclusions: In routine diagnosis of primary and secondary glomerulopathies, light microscopy and immunofluorescence and electron microscopy together present gold standard for pathological diagnosis. For complete diagnosis of glomerulopaties, pathologists from Split will use futher an electron microscope from Zagreb