Efficiency of newly synthesized oxime K048 and its effect on oxidative stress in rats poisoned by tabun

U ovom diplomskom radu ispitana je djelotvornost novosintetiziranog oksima K048 kao mogućeg antidota i njegov učinak na oksidacijski stres u štakora trovanih tabunom. Nakon određivanja akutne toksičnosti tabuna i oksima K048 na štakorima, skupine od po 4 životinje podvrgnute su različitim tretmanima. Kontrolnim životinjama aplicirana je fiziološka otopina (2 mg kg-1). Ostale skupine tretirane su tabunom (¾ LD50), oksimom K048 (¼ LD50) te atropinom (10 mg kg-1) ili kombinacijom oksim plus atropin nakon trovanja tabunom. U prvih 6 h od tretiranja štakora tabunom dobivene su niske aktivnosti ChE što ukazuje na njegov jak inhibitorni učinak. Značajan reaktivacijski učinak K048 uočen je povećanjem aktivnosti ChE u 30 min i 1 h nakon tretmana. Kao mogući doprinos toksičnosti tabuna smatra se uočeno povećanje razine TBARS (marker oksidacijskog stresa). K048 ne inducira oksidacijski stres te pokazuje nisku akutnu toksičnost i dobra terapeutska svojstva.In this graduate thesis, the efficiency of newly synthesized oxime K048 as potential antidote, and its effects on oxidative stress in rats poisoned by tabun. were examined. After determining of acute toxicity of tabun and oxime K048 on rats, groups of 4 animals to different treatments were submitted. To the control group animals a physiological solution (2 mg kg-1) was applied. Other groups of animals with tabun (¾ LD50), oxime K048 (¼ LD50) and atropine (10 mg kg-1) or with combinating oxime plus atropine after tabun poisoning were treated In first 6 hours since treating with tabun has happened, low activities od ChE were achieved which shows on its strong inhibiting effect. Significant reactivation effect of K048 was noticed by increased activity of ChE at 30 min and 1 hour after treatment. As possible contribution to tabun toxicity, one considers noticed increasing level of TBARS (oxidative stress indicator). K048 does not induce oxidative stress and shows low acute toxicity and good tearepeutic qualities