Application of response surface methodology in optimization of lignocellulosic materials enzymatic hidrolization

Istraživanje se temelji na poboljšanju procesa enzimske hidrolize pulpe šećerne repe (PŠR) s ciljem povećanja oslobađanja šećera. Komercijalni enzimski preparat (Viscozyme L) i statističke metode primijenjeni su za optimizaciju procesa hidrolize. Metoda odzivnih površina (RSM) je korištena za optimiziranje procesnih parametara nakon hidrolize. Najviša koncentracija šećera, 7,80 g/L, dobivena je pri pH 4, temperaturi 0 te 80 L enzima/g s.tv. PŠR.The investigation aims to enhance enzymatic sugar beet pulp (SBP) hydrolytic process for maximizing the sugar liberation. Commercial enzyme preparation (Viscozyme L) and statistical tools were applied to optimize hydrolysis process. Response surface methodology (RSM) was used for process parameters optimisation after hidrolization. The higher yield 7,80 g/L was obtain ith p , temperature 0 and 80 μL of enzyme/g d.m. SBP