Effect of laser on the sealing ability of bioactive retrofilling materials

Svrha ovog in vitro istraživanja je bila usporediti kvalitetu apikalnog brtvljenja mineral trioksid agregata (MTA) i biokeramičkog punila (Bioceramic, Root Repair Material, BC-RRM) u kavitetima izrađenim Er, Cr:YSGG laserom i UZV-om. U istraživanju je korišteno 48 humanih, ekstrahiranih jednokorijenskih zuba. Nakon ProTaper Universal tehnike instrumentacije, apikalni dijelovi korijenova su odrezani za 3 mm. Uzorci su nasumično podijeljeni dvije skupine ovisno o tehnici izrade retrogradnog kaviteta: Skupina 1: Er, Cr:YSGG laser, Skupina 2: ultrazvučna tehnika. U svakoj skupini, retrogradni kaviteti su u polovine uzoraka napunjeni s MTA-om, a u druge polovine s BC-RRM-om. U pozitivnoj kontrolnoj skupini retrogradni kaviteti su napunjeni s privremenim materijalom, a u negativnoj ružičastim voskom te je čitava površina premazana lakom. Uzorci su fiksirani u plastične Eppendorf epruvete i sterilizirani u plazmi. Korijenski kanali su kontaminirani suspenzijom E. faecalis-a, a epruvete napunjene s fetalnim bovinim serumom. Nakon 30 dana inkubacije, iz korijenskih kanala su prikupljeni mikrobiološki uzorci za metodu culture i test reakcije lančane polimeraze (PCR). Nije bilo značajne razlike između BC-RRM i MTA materijala u kavitetima izrađenim ultrazvučnim nastavkom (p > 0,05). Slične rezultate pokazao je MTA u kavitetima izrađenim Er, Cr:YSGG laserom (p > 0,05). Značajno veći broj bakterija otkriven je u skupini BC-RRM materijala punjenog u kavitetima izrađenim Er, Cr:YSGG laserom (p < 0.001). Nema razlike u kvaliteti apikalnog brtvljenja MTA materijala u retrogradnim kavitetima izrađenim Er, Cr:YSGG laserom i UZV-om. Najveće apikalno propuštanje zabilježeno je u kavitetima izrađenim laserom i punjenim BC-RRM-om.The aim of the in vitro study was to compare the apical sealing ability of Total Fill Bioceramic Root Repair Material (BC-RRM) and Mineral Trioxide Aggregate (MTA) regarding the retrogade preparation technique used: ultrasonic and Er, Cr:YSGG laser. The study sample consisted of 48 human single-rooted teeth, which were instrumented with ProTaper Universal rotary technique. After root-end resection (3 mm), the root specimens were divided in two groups according the retrogade preparation technique used: Group 1: ultrasonic technique, Group 2: Er, Cr :YSGGlaser. In each group, the half of the retrogade cavities was filled with BC RRM and another half with MTA The positive controls were filled with temporary material. In the negative controls, with utility wax. The outer root surfaces were covered with layers of nail varnish. The specimens were mounted in tubes and sterilized in plasma. The root canals were inoculated with E. faecalis suspension, and the tubes were filled with fetal bovine serum. After 30 days, the canals were sampled, cultured and the colony forming units (CFUs) were counted. The presence of E. faecalis was also determined by PCR. There was no significant difference between BC-RRM i MTA in the ultrasonic groups (p > 0,05). Similar results showed MTA in cavities made with Er, Cr:YSGG laser (p> 0.05). A significantly higher number of bacteria was detected in the laser group with BC-RRM filling (p