Production of ethanol on lignocellulosic raw hydrolyzate by Saccharomyces cerevisiae in a horizontal rotating tubular bioreactor

Proizvodnja biogoriva iz obnovljivih izvora postaje moguća alternativa fosilnim gorivima. Lignoceluloza je najzastupljeniji organski materijal na zemlji i jeftina obnovljiva sirovina za proizvodnju biogoriva. Međutim, zbog složene strukture, lignocelulozna biomasa mora biti podvrgnuta predobradi, te hidrolizi polisaharida na monosaharide koje mikroorganizmi mogu konvertirati u svom metabolizmu. U ovom istraživanju provedena je proizvodnja etanola na hidrolizatima lignoceluloznih sirovina (pšenična slama, kukuruzovina, kukuruzni oklasci) pomoću kvasca S. cerevisiae u horizontalnom rotirajućem cijevnom bioreatktoru. Ispitivano je i inhibitorno djelovanje furfurala na rast kvasca i proizvodnju etanola, te je utvđeno da koncentracija do 0,5 gL-1 značajno ne utječe na anaerobni uzgoj kvasca S.cerevisiae. Najveća uspješnost proizvodnje etanola zabilježena je kod hranjive podloge s hidrolizatom pšenične slame pri čemu su dobiveni ovi pokazatelji uspješnosti: Pr = 0,0678 gL-1h-1; Yp/s= 0,216 gg-1 i E= 42 %.Production of biofuels from renewable resources is becoming promising alternative to fossil fuels. Lignocellulose is the most abundant organic material on earth and cheap renewable resource for biofuel production. However, due to complex structure, lignocellulosic biomass has to be subjected to pretreatment and hydrolysis of polysaccharides to monosaccharides, which can be assimilated by microorganisms. This research was conducted ethanol production on hydrolysates of lignocellulosic raw materials (wheat straw, corn stalks, corn maize cobs) using the yeast S. cerevisiae in a horizontal rotating tubular bioreactor. It is examined the inhibitory effects of furfural on the growth of yeast and production of ethanol, and it was established that the concentration of up to 0.5 gL-1 does not significantly affect the anaerobic cultivation of the yeast S.cerevisiae. Best-ethanol production was observed in culture media with the hydrolyzate of wheat straw were obtained with these performance indicators: Pr = 0.0678 gL-1h-1; Yp/s = 0.216 gg-1, and E = 42%