Qualitative and quantitative analysis of polyphenols of Geranium macrorrhizum L. and G. dalmaticum (Beck) Rech. f., Geraniaceae

U okviru ovoga diplomskog rada provedena je kvalitativna i kvantitativna analiza polifenola vrsta Geranium macrorrhizum L. i G. dalmaticum (Beck) Rech. f. (Geraniaceae). Prisutnost polifenolnih tvari dokazana je općim reakcijama stvaranja obojenih produkata ili taloga. Kvantitativna analiza ukupnih polifenola (UP), trjeslovina (T), flavonoida (F) i fenolnih kiselina (FK) provedena je spektrofotometrijskim metodama. Sadržaj analiziranih polifenolnih tvari za vrstu Geranium macrorrhizum iznosio je: 21,55±0,40% (UP), 15,77±0,50% (T), 0,55±0,00% (F) i 1,53±0,05% (FK). Sadržaj analiziranih polifenolnih tvari za vrstu Geranium dalmaticum iznosio je: 21,76±0,07% (UP), 15,58±0,05% (T), 0,51±0,01% (F) i 1,33±0,13%. (FK). Provedena kvalitativna i kvantitativna analiza predstavlja prilog znanstvenom istraživanju vrsta roda Geranium i upotpunjuje dosadašnje spoznaje o fitoterapijskom potencijalu vrsta G. macrorrhizum i G. dalmaticum, posebice u odnosu na sadržaj bioaktivnih polifenolnih tvari.In this work, qualitative and quantitative analysis of polyphenols of Geranium macrorrhizum L. and G. dalmaticum (Beck) Rech. f. (Geraniaceae) was carried out. The presence of polyphenols was proven by using general reactions of developing colored products and precipitates. Quantitative analysis of total polyphenols (TP), tannins (T), flavonoids (F), and phenolic acids (PA) was carried out using spectrophotometric methods. The contents of analyzed polyphenolic compounds for Geranium macrorrhizum were as follows: 21.55±0.40% (TP), 15.77±0.50% (T), 0.55±0.00% (F), and 1.53±0.05% (PA). The contents of analyzed polyphenolic compounds for Geranium dalmaticum were as follows: 21.76±0.07% (TP), 15.58±0.05% (T), 0.51±0.01% (F), and 1.33±0.13%. (PA). Performed qualitative and quantitative analysis is a contribution to the scientific study of genus Geranium and completes the existing knowledge about phytotherapeutic potential of species G. macrorrhizum i G. dalmaticum, especially in relation to the content of bioactive polyphenolic substances