Chapter Twenty Seven - Generation of immune globulin single variable domains by display technologies36931

H.J. de Haard
A. Hultberg
T. Verrips
P. Vanlandschoot
M. Saunders
C. Stortelers
S. Van Gucht
C. Rupprecht
T. Nagarajan

Publication date

January 2014

DOI

10.1016/B978-0-12-800014-4.00027-5

Publisher

Elsevier BV

Abstract

Rabies virus-specific, cross-neutralizing, llama-derived heavy-chain antibody fragments (VHH) can be generated rapidly from immune llamas using phage display technology. The selection procedure can be tailor-made depending on the epitope to be targeted, whereas the single-chain nature of the VHH allows for facile genetic fusion of different VHH to create multivalent VHH. These VHH can target one or several different epitopes, thereby increasing the chance of neutralization of different strains of virus</p