Ion-chromatographic determination of glyphosate

Kromatografske metode su među najvažnijim metodama u analitičkoj kemiji jer omogućuju istovremeno odjeljivanje, identifikaciju i kvantitativno određivanje sastojaka u složenim smjesama. Ionska kromatografija (IC), često primjenjivana u industriji, vrlo je važna za analiziranje uzoraka koji sadrže ionske čestice. Najnoviji trendovi u kromatografiji općenito, pa time i u ionskoj, vezani su uz vezanje (uparivanje) više kromatografskih sustava u svrhu poboljšanja osjetljivosti analiza, ali i eventualne uštede troškova. Raspon analita je vrlo širok, od jednostavnih anorganskih iona do organskih molekula poput herbicida glifosata kojim se bavi i ovaj rad. Glifosat, često upotrebljavani herbicid, a koji može biti opasan po ljudsko zdravlje, u ovom je radu analiziran ionskom kromatografijom. Pri tome su za analizu primijenjeni 1D i 2D IC sustav. Za oba sustava razvijene su i validirane metode kromatografske analize glifosata. Primijenjena 1D metoda pokazala se nedostatnom za analizu realnog uzorka piva. 2D IC analizom uspješno je detektirana i kvantificirana koncentracija glifosata u analiziranom uzorku piva.Chromatographic methods are among the most important analytical methods since they enable simultaneously separation, identification and quantification of diverse compounds from complex matrices. Ion chromatography, often applied in industry, is very important for the analysis of samples containing ionic particles. Current trends in chromatography are related to multidimensional chromatography where two or more chromatographic systems are paired and applied for analysis in order to improve the method sensitivity and potentially reduce the analysis cost. Accordingly, same trends can be found in ion chromatography (IC) also. The range of IC analytes is very wide: from simple inorganic ions to organic molecules like herbicide glyphosate which is the analyte in this work. Glyphosate is very often used herbicide which can be dangerous to human health. This work describes development and validation of IC method for analysis of glyphosate in beer. 1D and 2D-IC systems were applied for this analysis. IC methods for glyphosate analysis were developed and validated for both systems. The applied 1D method proved to be insufficient for beer sample analysis. The applied 2D-IC method successfully detected and quantified glyphosate in analysed beer sample