The composition of phenols in blackthorn fruit during the ripening

U plodovima trnine različitih stupnjeva zrelosti određen je fizikalno-kemijski sastav, te je utvrđeno da gotovo u potpunosti sadrže reducirajuće šećere. Promjene fenolnih spojeva, i to posebno u pokožici i pulpi, određene su upotrebom HPLC s UV-VIS Diode Array detekcijom.. Nezreli plodovi trnine imali su najveći udio fenolnih spojeva i to u pulpi, te se taj udio smanjivao tijekom zrenja. U pokožici se količina fenolnih spojeva povećavala dozrijevanjem. Kroz sve faze zrelosti najzastupljeniji fenolni spojevi bili su neoklorogenska kiselina, klorogenska kiselina i kvercetin-3-ramnozid. Flavonoli su određeni u najvišim koncentracijama u srednje zrelim plodovima. Koncentracije flavonola određene u pokožici i u pulpi, neznatno su se razlikovale. Iz skupine antocijana određeni su cijanidin-3-glukozid, cijanidin-3-rutinozid i pelargonidin-3-glukozid. Pelargonidin-3-rutinozid nije pronađen u pokožici i pulpi nezrelih plodova trnine. Najviša antioksidativna aktivnost određena je u pulpi trnine srednje zrelosti.Physical and chemical analysis was made in blachtorn fruit at different stages of ripening and consistance of mainly reducing sugars was fortified. Alteration of polyphenols was determined in peel and pulp, by using HPLC with UV-VIS Diode Array detection. Immature fruits showed the highest amounts of polyphenols, esspecially in pulp, where the amount decreased during the ripening. The amount of polyphenols increased during the ripening in pulp. Dominant polyphenols at all ripening stages were neochlorogenic acid, chlorogenic acid and quercetin-3-ramnoside. The highest amounts of flavonols were determinated in semi-mature fruits. Concentrations of flavonols which were determinated in peel and pulp showed insignificant differences in values. Anthocyanines were determinated as cyandin-3-glucoside, cyandin-3-rutinoside and pelargonidine-3-glucoside. Pelargonidine-3-rutinoside was not found in peel and pulp of immature blackthorn. The highest antioxidant activity was determinated in pulp of semi-mature blackthorn