Effects of garlic extracts (Allium sativum) on normal cells growth and their antioxidative activity

Ciljevi istraživanja: Cilj rada bio je ispitati antioksidativni kapacitet i djelovanje 5 ekstrakata češnjaka na rast normalnih staničnih linija te provjeriti utječe li razlika u načinu proizvodnje na ukupnu aktivnost češnjaka. Nacrt studije: Pokusno (eksperimentalno) istraživanje Materijali i metode: Ekstrakti češnjaka pripremljeni su primjenom ultrazvučne tehnologije. Dva uzorka dobivena su komercijalno, dva uzorka dobivena su organski, a jedan je uzgojen na Poljoprivrednom fakultetu u Osijeku. Korištene su dvije normalne stanične linije (MDCK i BJ) kultivirane u CO2 inkubatoru (5% CO2, 37 ͦ C). Proliferativni učinak ekstrakta češnjaka ispitan je primjenom MTT testa dok je antioksidativna aktivnost određena primjenom DPPH metode. Obje su metode kolorimetrijske, a rezultati su očitani spektrofotometirijski. Primijenjen je protokol prilagođen za određivanje u mikrotitarskoj ploči. Rezultati: Ekstrakt češnjaka broj 2 ima najpozitivniji učinak na preživljenje obje stanične linije. Neki ekstrakti češnjaka pri koncentraciji od 4 mg/ml djeluju citotoksično na BJ staničnu liniju. Ekstrakti češnjaka ne pokazuju dostatan antioksidativni kapacitet pri ispitivanim koncentracijama od 0.01 mg/ml do 5 mg/ml. Zaključak: Citotoksično djelovanje ekstrakta češnjaka ovisno je staničnoj liniji. Iako svi ekstrakti pokazuju antioksidativna svojstva, antioksidativni kapacitet ekstrakta ovisi o načinu proizvodnje češnjaka.Objectives: The aim was to test the antioxidant capacity and the influence of five exstracts of garlic on the growth of normal cell lines and to check if the differences in manufacturing affect antioxidant activity in total. Study design: Experimental study Materials and methods: Extracts of garlic have been prepared by ultrasound technology. Two samples were bought in a store, two samples were grown organically and one is grown at the Faculty of agriculture in Osijek. There are two normal cell lines that were used (MDCK and BJ) and were cultivated in the CO2 incubator (5% of CO2, 37 ͦ C). Proliferative effect of garlic extracts was tested by applying MTT test while an antioxidant activity was detected by dint of DPPH method. The both methods were colorimetric and the results were detected by spectrofotometric reader. A modified protocol for a microplate was applied. Results: The second extract of garlic has the most positive effect on surviving of both cell lines. Some of the extracts have cytotoxic influence to the BJ cell line, especially at the concentration of 4 mg/mL. Extracts of garlic do not show sufficient antioxidative capacity at tested concentration from 0.01 mg/mL to 5 mg/mL. Conclusion: Cytotoxic effects of extracts of garlic depend on the kind of cell line. Even though all of the extracts have shown antioxidative activity, antioxidative capacity of garlic extract depends on the way garlic was produced