In vitro effect of soy hydrolysate on proliferation and morphology of HeLa and HepG2 tumor cell lines

Kulture životinjskih stanica uzgajane u hranjivom mediju sa serumom kao jednom od glavnih komponenti danas se koriste u gotovo svim područjima biotehnologije. Zbog brojnih nedostataka primjene seruma poput kemijske nedefiniranosti i mogućnosti kontaminacije, nastoji mu se pronaći prikladna zamjena. U tu se svrhu istražuje uloga proteinskih hidrolizata, od kojih su u fokusu najviše hidrolizati pšenice i soje. U ovom radu ispitan je in vitro učinak hidrolizata soje u koncentracijama od 1-5 g L-1 na proliferaciju (metoda tripan-plavo i MTT) i morfologiju (bojanje stanica kristal-ljubičastim) dviju humanih tumorskih staničnih linija HeLa i HepG2. Na temelju dobivenih rezultata može se zaključiti da hidrolizat soje pokazuje inhibitoran učinak na proliferaciju i morfologiju tretiranih stanica, ovisno o ispitanoj koncentraciji. Nadalje, dobiveni antiproliferacijski učinak je poželjan s aspekta terapije raka te bi trebalo istražiti koji su spojevi iz hidrolizata odgovorni za inhibiciju rasta tumorskih stanica kao i mehanizme njihova djelovanja.Animal cell cultures cultivated in culture medium containing serum as one of it's main components are used today in nearly every field of biotechnology. Because of many disadvantages of application of serum such as chemicaly undefined composition and possibility of contamination, there is an active search for it's suitable replacement. Protein hydrolysates are more and more examined for this purpose, and the main focus is on wheat and soybean hydrolysates. In this work, the in vitro effect of soy hydrolysate in a concentration of 1 to 5 g L-1 on the proliferation (trypan blue and MTT methods) and morphology (cristal violet cells colouring) of the two human tumor cells; HeLa and Hep2G, was examined. Based on the results it can be concluded that soy hydrolysate shows generally an inhibiting effect on the survival and growth of treated cells, depending on examined concentration. Furthermore, the obtained antiproliferative effect is desirable in terms of treatment of cancer and hydrolysates compounds responsible for tumor cell growth inhibition as well as mechanisms of their action should be examined