Preparation of imidazolium ionic liquids and their application in biotransformations

Cilj ovog rada bila je sinteza imidazolijevih ionskih tekućina te njihovo ispitivanje kao novih medija za biotransformacije. Ionske tekućine pripravljene su reakcijama kvaternizacije 1,2-dimetilimidazola s 1-brompentanom, 1-bromheksanom i 1-bromheptanom te reakcijama izmjene aniona s litijevim bis[(trifluormetil)sulfonil]imidom. Procesi sinteze provedeni su šaržno, klasičnim postupcima organske sinteze te kontinuirano u mikroreaktorskom sustavu s mikromješačem. Novosintetizirane ionske tekućine karakterizirane su 1H- i 13C-NMR-om. Također, ionske tekućine ispitane su kao novi mediji u reakciji sinteze izoamil-acetata s imobiliziranom lipazom B izoliranom iz kvasca Candida antarctica (Novozym 435), a za usporedbu, reakcija je provedena u komercijalnoj ionskoj tekućini [BMIm][Tf2N].The aim of this study was the synthesis of imidazolium ionic liquids and their testing as new media for biotransformations. Ionic liquids were prepared by quaternization of 1,2-dimethylimidazole with 1-bromopentane, 1-bromohexane and 1-bromoheptane and anion exchange reactions with lithium bis[(trifluoromethyl)sulfonyl]imide. The syntheses were carried out by batch conventional organic synthesis methods, and continuously in a micro reactor system with micro mixer. The novel ionic liquids were characterized by 1H-and 13CNMR spectra. Also, ionic liquids have been examined as new media for the isoamyl acetate synthesis with immobilized lipase B isolated from Candida antarctica (Novozym 435). For the comparison the reaction in commercial ionic liquid [BMIm][Tf2N] was carried out