Comparison of two solid-state bioreactor configurations for white-rot fungus Trametes versicolor cultivation

U ovom radu gljiva bijelog truljenja Trametes versicolor uzgajana je u uvjetima fermentacije na čvrstim nosačima u dva različita laboratorijska bioreaktorska sustava (horizontalno-cilindrični stakleni reaktor-HBR i laboratorijski biorektor s pliticama-TBR) s ciljem istraživanja uspješnosti biološke obrade lignoceluloznog materijala. U oba sustava, kao supstrat su korišteni repini rezanci identičnog sastava, početne mase i vlažnosti, dok je kao inokulum korišten isti broj micelijskih diskova čiste kulture T. versicolor TV-6. Oba sustava bila su aerirana na istovjetan način.U horizontalnom bioreaktoru miješanje je provođeno periodično, dok je uzorak u bioreaktoru s pliticama nanesen u tankom sloju te je kao takav bio u statičnim uvjetima tijekom aerobne fermentacije. Tijekom 30 dana fermentacije praćeni su relevantni procesni parametri (temperatura u različitim slojevima supstrata, relativna vlažnost) te gubitak na masi i promjena boje uzorka. Nadalje, određivan je kemijski sastav lignoceluloznog materijala (udjeli vode, pepela, celuloze i pentozana).Primjenom oba sustava postignut je gubitak na masi lignoceluloznog materijala (21,33 % HBR i 46,50 % TBR), povećanje udjela pepela (175,5 % HBR i 78,9 % TBR), smanjenje udjela celuloze (64,65 % HBR i 42,34 % TBR) te smanjenje udjela pentozana (42,39 % HBR i 62,61 % TBR). Dobiveni rezultati upućuju na mogućnost primjene oba sustava za razgradnju i/ili prethodnu obradu lignoceluloznih materijala pomoću gljive bijelog truljenja T. versicolor.In this study, white-rot fungus Trametes versicolor was cultivated under solid state conditions using two different laboratory bioreactors (horizontal cylindrical glass bioreactor and tray bioreactor). The aim of the study was to investigate the biological treatment efficiency of lignocellulosic material. Sugar beet waste of identical chemical composition, initial mass and moisture content was used as a substrate, while the same amount of T. versicolor TV-6 mycelial discs were used as inoculum in both bioreactors. Furthermore, both bioreactors were aerated identically. Bed layer mixing in horizontal bioreactor was performed periodically, while inoculated substrate in the tray bioreactor remained statical throughout the experiment. During 30 days of fermentation, relevant process parameters (temperature in different bed layers and relative moisture) were measured and chemical composition of substrate (% of moisture, ash, cellulose and pentosane), weight loss and substrate color change were determined. Material weight loss (21,33% HBR and 46,50% TBR), ash content increase (175,5% HBR and 78,9% TBR), cellulose content decrease (64,65% HBR and 42,34% TBR) as well as pentosane content (42,39% HBR and 62,61% TBR) were observed in both investigated bioreactor systems. The obtained results indicate that both bioreactor systems can be used for biological degradation and/or pretreatment of lignocellulosic materials using white-rot fungus T. versicolor as inoculum