(Figure Presented) A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flow-cell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived. \ua9 2009 Wiley-VCH Verlag GmbH & Co. KGaA
Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases ...
Proteomic studies of plasma membrane proteins are challenged by the limited solubility of these prot...
Despite their importance in many biological processes, membrane proteins are underrepresented in pro...
Plasma membrane (PM) proteins play crucial roles in cell signaling and communications, and they are ...
<p><b>Copyright information:</b></p><p>Taken from "Proteomic analysis of plasma membrane and secreto...
<p><b>Copyright information:</b></p><p>Taken from "Proteomic analysis of plasma membrane and secreto...
Advancing the quest for new drug targets demands the development of innovative plasma membrane prote...
We manufactured a novel type of lipid-coated superparamagnetic nanoparticles that allow for a rapid ...
The development of fully automated and high-throughput systems for proteomics is now in demand becau...
Membrane proteins play important roles in various plasma membrane (PM) activities such as signal tra...
All techniques needed for proteomic analyses of plant plasma membranes are described in detail, from...
<p><b>Copyright information:</b></p><p>Taken from "Proteomic analysis of plasma membrane and secreto...
A major goal of proteomics is to develop methods that enable the systematic characterization of ever...
Proteins residing in the plasma membrane have key functions in transport, signal transduction, vesic...
Membrane proteins (MP) play an important role in biological processes. Isolation and quantification ...
Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases ...
Proteomic studies of plasma membrane proteins are challenged by the limited solubility of these prot...
Despite their importance in many biological processes, membrane proteins are underrepresented in pro...
Plasma membrane (PM) proteins play crucial roles in cell signaling and communications, and they are ...
<p><b>Copyright information:</b></p><p>Taken from "Proteomic analysis of plasma membrane and secreto...
<p><b>Copyright information:</b></p><p>Taken from "Proteomic analysis of plasma membrane and secreto...
Advancing the quest for new drug targets demands the development of innovative plasma membrane prote...
We manufactured a novel type of lipid-coated superparamagnetic nanoparticles that allow for a rapid ...
The development of fully automated and high-throughput systems for proteomics is now in demand becau...
Membrane proteins play important roles in various plasma membrane (PM) activities such as signal tra...
All techniques needed for proteomic analyses of plant plasma membranes are described in detail, from...
<p><b>Copyright information:</b></p><p>Taken from "Proteomic analysis of plasma membrane and secreto...
A major goal of proteomics is to develop methods that enable the systematic characterization of ever...
Proteins residing in the plasma membrane have key functions in transport, signal transduction, vesic...
Membrane proteins (MP) play an important role in biological processes. Isolation and quantification ...
Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases ...
Proteomic studies of plasma membrane proteins are challenged by the limited solubility of these prot...
Despite their importance in many biological processes, membrane proteins are underrepresented in pro...
DOI
Add to ORKG