Enzymatic Hydrolysis of Different Cellulosic and Lignocellulosic Materials: What are the Limiting Factors?

Lignocellulose is the most abundant renewable material on earth and plays an important role in development of sustainable energy industry and production of value-added products. Hydrolysis of lignocellulosic materials is most often performed by enzymes produced by the filamentous fungus Trichoderma reesei, which is the main industrial source for enzyme production. However, hydrolysis of lignocellulose is usually incomplete, but limitations are not well understood. We studied the enzyme profile produced during the fungal growth on model cellulosic and lignocellulosic substrates and their capacity to hydrolyze cellulosic and lignocellulosic substrates with different chemical and physical properties. The results of our study will bring insight into bottlenecks of enzymatic hydrolysis