Studies of glycolytic flux regulation/control using permeabilised yeast cells

In this work the correlation between levels of adenosine nucleotides and glycolytic flux was studied using a permeabilised cell system by experiments and in silico simulations using a kinetic model of glycolysis. Experimentally, using a flow-through chamber, the ATP level was correlated to glycolytic flux by a parabolic relation with a maximum at 2-3 mM of ATP. The kinetic model did not describe this parabolic correlation; at high ATP levels above 4 mM the flux was further increased. Thus, other mechanisms than described by the kinetic equations used in the model are important at high ATP levels to reduce the glycolytic flux