Relative protein abundances of Escherichia coli MG1655 growing exponentially on minimal medium with acetate or glucose as the sole carbon source were investigated in a quantitative shotgun proteome analysis with TMT6-plex isobaric tags. Peptides were separated by high resolution high/low pH 2D-LC, using an optimized fraction pooling scheme followed by mass spectrometric analysis. Quantitative data were acquired for 2099 proteins covering 49% of the predicted E. coli proteins, showing system-wide effects of growth conditions. In total, 507 proteins showed a fold change of at least 1.5 and 205 proteins changed by more than twofold. Significant differences in abundance were observed for most of the proteins in the central carbon metabolism and...
International audienceMetabolic engineering aims to design high performance microbial strains produc...
AbstractEscherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and t...
Modern systems biology requires extensive, carefully curated measurements of cellular components in ...
Abstract Accurate measurements of cellular protein concentrations are invaluable to quantitative stu...
Accurate measurements of cellular protein concentrations are invaluable to quantitative studies of g...
Measuring precise concentrations of proteins can provide insights into biological processes. Here we...
Measuring precise concentrations of proteins can provide insights into biological processes. Here we...
Protein mass is a major constituent of bacterial cell dry weight. An exponentially growing cell dive...
Knowledge about the abundance of molecular components is an important prerequisite for building quan...
analysis was used to compare global protein expression changes in Escherichia coli fermenta-tion bet...
Here we describe a proteomic analysis of Escherichia coli in which 3,199 protein forms were detected...
The costs and benefits of protein expression are balanced through evolution. Expression of un-utiliz...
We describe a novel LCMS approach to relative quantitation and simultaneous identification of protei...
The proteome reflects the available cellular machinery to deal with nutrients and environmental chal...
Background: The proteome reflects the available cellular machinery to deal with nutrients and enviro...
International audienceMetabolic engineering aims to design high performance microbial strains produc...
AbstractEscherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and t...
Modern systems biology requires extensive, carefully curated measurements of cellular components in ...
Abstract Accurate measurements of cellular protein concentrations are invaluable to quantitative stu...
Accurate measurements of cellular protein concentrations are invaluable to quantitative studies of g...
Measuring precise concentrations of proteins can provide insights into biological processes. Here we...
Measuring precise concentrations of proteins can provide insights into biological processes. Here we...
Protein mass is a major constituent of bacterial cell dry weight. An exponentially growing cell dive...
Knowledge about the abundance of molecular components is an important prerequisite for building quan...
analysis was used to compare global protein expression changes in Escherichia coli fermenta-tion bet...
Here we describe a proteomic analysis of Escherichia coli in which 3,199 protein forms were detected...
The costs and benefits of protein expression are balanced through evolution. Expression of un-utiliz...
We describe a novel LCMS approach to relative quantitation and simultaneous identification of protei...
The proteome reflects the available cellular machinery to deal with nutrients and environmental chal...
Background: The proteome reflects the available cellular machinery to deal with nutrients and enviro...
International audienceMetabolic engineering aims to design high performance microbial strains produc...
AbstractEscherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and t...
Modern systems biology requires extensive, carefully curated measurements of cellular components in ...