Toward the analysis of mitochondria isolated from leukemic cells with electrochemically instrumented microwell arrays
- Lemercier, Gabriel
- Sekli-Belaidi, Fadhila
- Vajrala, Venkata,
- Descamps, Emeline
- Sojic, N.
- Arbault, Stéphane
- Sarry, Jean Emmanuel,
- Temple-Boyer, Pierre
- Launay, Jérôme
Publication date
June 2017
DOI Publisher
Institute of Electrical and Electronics Engineers (IEEE)Abstract
International audienceThis work deals with the development of electrochemical transducers for the analysis of the metabolic status of mitochondria isolated from leukemic cells. It proposes the use of ring nanoelectrodes (RNE) integrated into microwell arrays for the simultaneous monitoring of the oxygen (O2) consumption and the hydrogen peroxide (H2O2) production. The sensor enabled the real-time recording of the oxygen consumption of approximately 10,000 isolated mitochondria. Solutions are now proposed to detect H2O2 production and to reduce the number of mitochondria under test, targeting the single mitochondrion analysis
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