Oxidation-reduction changes in the rat brain induced by isoflurane and iron-dextran

Inhalacijski anestetici normalno se smatraju nereaktivnim, ali pod odgovarajućim uvjetima metaboliziraju se u reaktivne slobodne radikale i stupaju u reakcije s dijelovima stanica pri čemu dovode do oštećenja. U mozgu željezo igra važnu ulogu u proizvodnji mijelina, metabolizmu monoaminskih transmitera i sintezi GABA. S druge strane, nakupljanje željeza u mozgu mijenja metabolizam stanica mozga i vodi povećanju oksidacijskog stresa te neurodegeneraciji. Cilj rada bio je istražiti mogući antioksidativni/prooksidativni učinak izoflurana i željezo-dekstrana, kao i njihovo zajedničko djelovanje na uzorcima tkiva mozga štakora: a) procjenom razine neuroupale, mjerenjem relativne težine mozga u odnosu na zdravu kontrolu; b) promjene u oksidacijsko-redukcijskom statusu mjerenjem razine malondialdehida (MDA, krajnjeg produkta lipidne peroksidacije) i glutationa (GSH), te aktivnosti katalaze (CAT) i superoksid dismutaze (SOD); c) analizom promjene hematoloških pokazatelja krvi, te osmotske fragilnosti eritrocita. Rezultati ukazuju da izofluran uzrokuje oksidacijski stres u mozgu, dok njegova kombinacija s Fe-dekstranom ima neuroprotektivno djelovanje. Fe-dekstran uzrokuje povećanu lipidnu peroksidaciju i neuroupalu. Izofluran i kombinacija izoflurana i Fe-dekstrana uzrokuje porast razine eritrocita te pad svih ostalih hematoloških parametara, dok sam Fe-dekstran uzrokuje pad svih hematoloških parametara.Inhaled anesthetics are normally considered non-active, but under appropriate conditions, they are metabolized in reactive free radicals and reacting with cellular parts, causing damage. In the brain, iron plays an important role in the production of myelin, metabolism of monoamine transmitters and GABA synthesis. On the other hand, the accumulation of iron in the brain changes brain cell metabolism and leads to increased oxidative stress and neurodegeneration. Aim of this study was to investigate the possible antioxidant/prooxidative effect of isoflurane and Fe-dextran, as well as their combined interaction on the rat brain tissue samples: a) evaluation of the neuro-inflammation, by measuring the relative weight of the brain compared to a healthy control group; b) changes in the oxido-reduction status by measuring the level of malondialdehyde (MDA, end product of lipid peroxidation) and glutathione (GSH), activity of catalase (CAT) and superoxide dismutase (SOD); c) analyzing changes in haematological parameters and osmotic fragility of erythrocytes. Results indicate that isoflurane causes oxidative stress in the brain, while its combination with Fe-dextran has neuroprotective action. Fe-dextran causes increased lipid peroxidation and neuro-inflamattion. Isoflurane and its combined usage with Fe-dextran causes increased levels of erythrocyte and decreased of all hematological parameters, while alone Fe-dextran causes decreased of all hematological parameters