The influence of growth location and harvest time on phenolic compounds in mastic tree leaves (Pistacia lentiscus L.)

Tršlja je vazdazelena biljka, karakteristična za mediteransko područje, a smatra se bogatim izvorom fenolnih spojeva. Cilj ovog istraživanja bio je odrediti utjecaj lokacije uzgoja i termina berbe na koncentraciju ukupnih fenolnih spojeva lista tršlje. Uzorci su brani sa 4 lokacije s područja Hrvatske u 2 termina berbe. Za provođenje ekstrakcije korištena je vodena otopina etanola, a maseni udjeli ukupnih flavonoida, hidroksicimetnih kiselina i flavonola određeni su spektrofotometrijskim metodama. Prema dobivenim rezultatima, najviše koncentracije ukupnih flavonoida, hidroksicimetnih kiselina i flavonola zabilježene su na Korčuli u prvom terminu berbe (8,71 mg/g uzorka, 9,85 mg/g uzorka i 9,13 mg/g uzorka). U drugom terminu berbe, na Korčuli je određena najniža koncentracija ukupnih flavonoida, hidroksicimetnih kiselina i flavonola (3,96 mg/g uzorka, 5,05 mg/g uzorka, 4,58 mg/g uzorka), dok je najviša koncentracija ukupnih flavonoida zabilježena na Hvaru (8,62 mg/g uzorka). Najviše koncentracije ukupnih hidroksicimetnih kiselina i flavonola u drugom terminu berbe određene su na Barbarigi (7,73 mg/g uzorka, 8,27 mg/g uzorka).Mastic tree is an evergreen plant, characteristic in the Mediterranean area and is considered as a rich source of phenolic compounds. The aim of this study was to determine the influence of the growth location and harvest time on the concentration of the total phenolic compounds in mastic tree leaves. Samples are collected from 4 locations in Croatia in 2 harvest periods. Aqueous ethanol solution was used for extraction, while mass concentrations of total flavonoids, hydroxycinnamic acids and flavonols were determined by spectrophotometric methods. According to the results, in the first harvest period, the highest concentration of total flavonoids, hydroxycinnamic acids and flavonols were found on Korčula (8,71 mg/g sample, 9,85 mg/g sample and 9,13 mg/g sample). In the second harvest period, the lowest concentrations of total flavonoids, hydroxycinnamic acids and flavonols (3,96 mg/g sample, 5,05 mg/g sample, 4,58 mg/g sample) were determined at Korčula, while the highest concentration of total flavonoids was found on Hvar (8,62 mg/g sample). The highest concentrations of total hydroxycinnamic acids and flavonols in the second harvest period were determined at Barbariga (7,73 mg/g sample, 8,27 mg/g sample)