Determination of neomycin in animal food

Zbog raširene primjene antibakterijskih lijekova, mora se kontrolirati prisutnost rezidua u hrani životinjskog podrijetla. Kontrola antibiotika u hrani potrebna je kao jedna od temeljnih odrednica u službi očuvanja zdravlja ljudi. Cilj ovog rada bio je odrediti koncentracije ostataka (rezidua) neomicina u mlijeku i mišićnom tkivu domaćih životinja. Koncentracija neomicina u uzorcima je određivana ELISA metodom. Prema dobivenim rezultatima, niti u jednom ispitanom uzorku određena koncentracija neomicina nije prelazila vrijednost najveće dopuštene količine (NDK) za neomicin propisane Uredbom Europske komisije 37/2010 o najvećoj dopuštenoj količini (NDK) ostataka antibiotika u hrani životinjskog podrijetla. Također je pokazano da je ELISA pozudana metoda za određivanje rezidua neomicina u uzorcima hrane životinjskog podrijetla. Da su uzorci bili pozitivni na neomicin, u tom bi slučaju bilo potrebno primjeniti jednu od metoda za potvrdu kojima se identitet rezidua i njegova koncentracija potvrđuju s najvećom mogućom preciznošću i točnošću, kao što su LC–MS/MS i GC–MS/MS.Because of the widespread use of antibacterial drugs, must be controlled the presence of residues in food of animal origin. Control of antibiotics in food is needed as one of the basic elements in the service of preserving the health of people. The aim of this study was to determine the concentrations of residues (residue) of neomycin in milk and muscle tissue of animals. Neomycin concentration in samples was determined by ELISA method. According to the results, in any sample examined the concentration of neomycin did not exceed the value of the maximum levels (MRLs) for neomycin prescribed in the Council Regulation 37/2010/EU on pharmacologically active substances and their classification regarding maximum residue limits in food stuffs of animal origin. It was also shown that the ELISA greedy method for determining residues of neomycin in food samples of animal origin. That the samples were positive to neomycin, in this case it would be necessary to apply one method to confirm that the identity of the residue and its concentration is confirmed with the utmost precision and accuracy, such as LC-MS/MS and GC-MS/MS