Content of bioactive compounds in root of stinging nettle (Urtica dioica l.)

Cilj ovog istraživanja bio je odrediti utjecaj gnojidbe tla dušikom na udio bioaktivnih spojeva (fenolni spojevi) u rizomu dvodomne koprive. Kao kontrolni uzorak korišten je rizom uzgojen na tlu koje nije tretirano gnojivom, a drugi rizom na tlu koje je tretirano gnojivom u količini 100 kg N/ha. Ekstrakcija ukupnih fenola provedena je primjenom destilirane vode i 50 %-tne vodene otopine etanola (v/v) uz primjenu ultrazvučne kupelji. Fenolni spojevi (ukupni fenoli, flavan-3-oli i hidroksicimetne kiseline) su određeni spektrofotometrijski. Utvrđene su veće koncentracije svih skupina fenolnih spojeva u rizomima koji su tretirani gnojivom u odnosu na netretirane.The aim of this study was to determine the influence of nitrogen fertilization on the content of bioactive compounds (phenolic compounds) in stinging nettle root. The controlled sample used was the cultivated stinging nettle root, which was not treated with nitrogen fertilization. The other sample of stinging nettle root was treated with a solution of 100 kg N/ha nitrogen fertilization. The extractions of total phenols was performed in ultrasound bath using distilled water and 50% aqueous ethanol (v/v) as extraction solvent. Phenolic compounds (total phenols, flavan-3-ols and hydroxycinnamic acid) were detected by spectrophotometric method. The higher amounts of bioactive compounds are determined in the stinging nettle root with 100 kg N/ha fertilization