The effect of 17β-Estradiol on proliferation of CCO I RTG-2 fish cell lines

Veliki broj prirodnih, farmaceutskih i sintetskih spojeva s estrogenim učinkom ispušta se u okoliš putem komunalnih i industrijskih otpadnih voda, pa izazivaju promjene rasta, razvoja, ponašanja i reproduktivnih sposobnosti organizama koji žive u vodi. U cilju određivanja i praćenja njihovih učinaka razvijeni su in vitro sustavi koji uključuju i testove citotoksicnosti sa stanicama riba. Stanicne linije CCO (Channel catfish ovary) i RTG-2 (Rainbow trout gonades) dobivene su iz primarne kulture ovarija mladog kanalskog soma, odnosno gonada juvenilne pastrve (Salmo giardneri) i primijenjene su u ispitivanju utjecaja 17β-estradiola na proliferaciju stanica. Na temelju provedenih istraživanja može se zaključiti da dodatak 17β-estradiola smanjuje proliferaciju CCO i RTG-2 staničnih linija proporcionalno koncentraciji. CCO i RTG-2 stanične linije su pogodne za primjenu u ekotoksikološkim ispitivanjima.Large number of natural, pharmaceutical and synthetic compounds with estrogen effect are released into the environment through municipal and industrial wastewater causing changes of growth, development and reproductive capacities for organisms who live in the water. To determine and monitor their effects, scientists developed in vitro systems which include tests of cytotoxicity with fish cells. CCO (Channel catfish ovary) and RTG-2 (Rainbow trout gonades) cell lines were obtained from the primary culture of Channel catfish ovary and Rainbow trout gonades and they were applied to study the effect of 17β-estradiol on cell proliferation. Based on the research it can be concluded that 17β-estradiol reduces proliferatin of CCO and RTG-2 cells showing concentration-dependenty. CCO and RTG-2 cell lines are suitable for use in ecotoxicology