DGAT1 gene polymorphism and fatty acid composition of fat in simmental beef cattle

Cilj rada bio je odrediti frekvencije alela i genotipova polimorfizma bovinog DGAT1 gena koji kodira diacilglicerol-O-aciltransferazu, enzim koji sudjeluje u završnom koraku sinteze triglicerida, i njegovu povezanost sa taloženjem masti i sastavom masnih kiselina masnog tkiva (loj) tovne junadi. DGAT1 K232A polimorfizam genotipiziran je PCR/RFLP metodom u DNA ekstrahiranoj iz uzoraka leđnog mišića 26 jednogodišnjih simentalskih bikova i junica iz intenzivnog uzgoja. K alel DGAT1 gena bio je manje zastupljen kod junica (0.08%) nego kod bikova (0.27%) sa ukupnom frekvencijom od 17% K alela. Ukupna genotipska frekvencija bila je viša za AA nego KA, dok genotip KK, povezan s jačim nakupljanjem masnog tkiva, nije pronađen. Nije utvrđeno odstupanja od Hardy-Weinbergove ravnoteže. Zamašćenje trupa i stupanj nezasićenosti masti bili su viši u junica nego u bikova, bez interakcije DGAT1 i spola. Utjecaj polimorfizma DGAT1 gena na nakupljanja i masno-kiselinski sastav masnog tkiva nije utvrđen.The aim of this study was to determine allele and genotype frequencies of bovine DGAT1 gene encoding the diacylglycerol-O-acyltransferase , enzym that catalyses the final step of triglyceride synthesis, and its association with deposition and fatty acid composition of fat tissue in fattening beef cattle. DGAT1 K232A polymorphism was genotyped with PCR/RFLP method from DNA extracted from longissimus muscle of 26 Simmental beef cattle from intensive production. K allele of DGAT1 gene was less frequent in heifers (0.08%) than in bulls (0.27%), with overall allelic frequency of 17% K allele. Overall genotype frequency was higher for AA than KA, while KK genotype, associated with increased accumulation of fat tissue, was not found. No deviation from Hardy-Weinberg equilibrium was found. Heifers showed a higher degree of carcass fattening and higher degree of unsaturation of fat than bulls; however, there was no interaction between sex and DGAT1 gene. Effect of DGAT1 gene polymorphism on deposition and fatty acid composition of fat tissue was not found