Evidence of presence of bacteria Listeria monocytogenes in fresh cheese by using cultivation and ELFA method

Svrha ovog rada bila je odrediti moguću prisutnost bakterije Listeria monocytogenes u uzorcima svježeg sira domaće i industrijske proizvodnje te obzirom na dobivene rezultate utvrditi njihovu mikrobiološku i zdravstvenu ispravnost prema mikrobiološkim kriterijima za hranu (NN 74/08). Posebna pažnja posvećena je dokazivanju prisutnosti bakterije L. monocytogenes u navedenim uzorcima, zbog njezine izrazite opasnosti po ljudsko zdravlje. Za mikrobiološka istraživanja korištena je metoda kultivacije kao predstavnik klasičnih metoda te metoda dokazivanja antigena spomenute bakterije metodom ELFA-e, na uređaju mini – VIDAS®, kao predstavnik alternativnih metoda. Od ukupno istraženih 80 uzoraka svi su potvrđeni kao zdravstveno i mikrobiološki ispravni s obzirom na istraživane parametre.The purpose of this study was to determinate possible presence of bacteria Listeria monocytogenes in samples of domestic and industrial fresh cheese and due to taken results define their microbiological and health correctness according to microbiological norms for food (OG 74/08). Particular attention is given to evidence of presence of bacteria L. monocytogenes in these samples, because of its exceptional hazards to human health. Method of cultivation, as official of conventional methods, and ELFA method on mini – VIDAS® instrument, as official of alternative methods, were used for microbiological analysis. All of 80 analyzed samples were confirmed as microbiologically and health correct due to researched parameters