Isolation of phenolic compounds from elderberry

Ekstrakcija je prvi i važan korak u izolaciji fenolnih spojeva i ne postoji jedinstveni standardni postupak ekstrakcije. U ovom radu provedena je ekstrakcija po Soxhlet-u, a dobiveni ekstrakt je pročišćen i razdvojen pomoću TLC na tri komponente koje su karakterizirane primjenom IR-spektroskopije čime je potvrđena prisutnost fenolnih spojeva u glogu. U ovom radu određena je i masena koncentracija ukupnih fenola u ekstraktu dobivenom ekstrakcijom po Soxhletu koja je iznosila 2166 mg GAE/100 g gloga što pokazuje uspješnost primjenjene metode ekstrakcije.Extraction is the first and important step in the isolation of phenolic compounds and there is no uniform standard extraction procedure. Extraction in this work was carried out by Soxhlet extraction. The resulting extract was purified and separated by TLC on three components that are characterized by IR spectroscopy confirming the presence of phenolic compounds in hawthorn. In this work was also determined the mass concentration of total phenols in the extract obtained by Soxhlet extraction. Mass concentration of total phenols in hawthorn totaled 2166 mg GAE/100 g hawthorn what indicates how successful the implemented method of extraction was