Molecular typing of Escherichia coli by pulsed-field gel electrophoresis

U svrhu epidemioloških analiza klinički mikrobiolozi dokazuju povezanost unutar grupe bakterijskih izolata, odnosno tipiziraju i dokazuju broj klonova unutar jedne bakterijske vrste. Cilj ovog rada bilo je molekularno tipiziranje dvije grupe izolata E. coli gel elektroforezom u pulsirajućem polju. To je metoda koja omogućuje razdvajanje velikih fragmenata DNA i do 10 Mb. Na temelju dobivenih rezultata određuje se sličnost među izolatima. U ovom radu također je testirana i osjetljivost ove dvije grupe izolata na standardne antibiotike, te je utvrđena razlika njihove osjetljivosti. Sukladno predviđanjima bolnički izolati pokazali su veću rezistenciju od vanbolničkih.Clinical microbiologists are often asked to determine the relatedness of a group of bacterial isolates in order to perform epidemiological analysis, that is, to type them and document the number of clones in one bacterial species. The point of this study was molecular identification of two groups of E. coli isolates by pulsed-field gel electrophoresis. This is a method that provides separation of large DNA fragments, up to 10 Mb and according to the results, the similitary between isolates can be determined. Also in this study, the susceptibility of these two groups of isolates to standard antibiotics was tested. Diference in susceptibility is determined. As expected, hospital isolates are proved to be more resistent than non hospital isolates