Oxidative Stability of Cow and Goat Milk after Intramammary Application of Propolis

Cilj ovog diplomskog rada bio je istraţiti utjecaj 1% i 5% bezalkoholne otopine propolisa primijenjene intramamarno u lijeĉenju supkliniĉkih mastitisa na antioksidacijska svojstva mlijeka krava i koza. Pripravak 1% nativnog propolisa apliciran je mlijeĉnim kravama trokratno u sve ĉetvrti vimena i to poslije jutarnje muţnje, poslije veĉernje muţnje te sljedeći dan poslije jutarnje muţnje. Koze ĉije je mlijeko bilo mikrobiološki pozitivno podijeli smo u dvije skupine. Jednoj skupini koza trokratno smo aplicirali intramamarno otopinu 5% nativnog propolisa, dok smo drugoj skupini trokratno aplicirali intramamarnu suspenziju antibiotika klavuxila. U mlijeku su odreĊene aktivnosti glutation peroksidaze, superoksid dismutaze, biološki antioksidacijski potencijal te koncentracija reaktivnih kisikovih metabolita, kao pokazatelja oksidacijskog statusa. U uzorcima mlijeka koza aktivnost GSHPx, SOD bila viša nego kod krava, a u uzorcima mlijeka koza koncentracija d-ROM nije bila mjerljiva. Aplikacija propolisa dovela je do porasta aktivnosti GSH-Px kao i porasta omjera GSH-Px/SOD u mlijeku koza nakon prve aplikacije te smanjenja koncentracije BAP u mlijeku obje vrste ţivotinja. Aplikacija dvije doze propolisa kod krava dovela je do smanjenja koncentracije d-ROM u mlijeku tretiranih ţivotinja dok se aktivnost SOD tijekom istraţivanja nije mijenjala. Nakon trokratne aplikacije 1% propolisa u ĉetvrti krava koje su bile zahvaćene supkliniĉkim mastitisom svi pretraţeni uzorci mlijeka bili su mikrobiološki negativni, a kod koza 73% pretraţenih uzoraka mlijeka bilo je mikrobiološki negativno. Aplikacijom propolisa dokazan je antioksidacijski i antimikrobni uĉinak propolisa apliciranog putem intramamarne formulacije.The aim of this study was to exemine effect of 1% and 5% non-alcoholic solution of propolis applied intramammary during treatment of subclinical mastitis on antioxidant properties of cow and goat milk. Solution of 1% native propolis was administered to milking cows three times in all four quarters of udder, after morning milking, after evening milking and next day after morning milking. Goats with positive microbiological findings was divided in two groups. First group was treated with 5% solution of native propolis intramammary three times, while the second group was treated with antibiotic klavuxil three times. Activities of glutathione peroxidase and superoxide dismutase, biological antioxidant potential and concentration of the reactive oxygen metabolites ware determined in milk. In samples of goat milk activity of GSH-Px and SOD was higher than in cow milk, while concentration of d- ROM in goat milk was not mesasurable. Application of propolis resulted in higher activity of GSH-Px and also higher ratio of GSH-Px/SOD in goat milk after first application, while in both goat and cow milk concentration of BAP was lower. Application of two doses of propolis in cows resulted in lower d-ROM concentration in milk of treated animals while activity od SOD during study show no changes. After applications of 1% propolis three times in udder quarters that were affected with subclinical mastitis, all samples of cow milk were microbiologically negative, while 73% of goat milk samples were microbiologically negative. Application of propolis confirmed antioxidant and antimicrobial effect of intramammary formulation of propolis