Barrier properties of the animal cornea after storage in commercially available mediums

S obzirom da je epitel rožnice glavna barijera prijenosu djelatnih tvari preko rožnice, očuvanje epitela rožnice važno je za provođenje uspješnih ex vivo ispitivanja permeabilnosti. U ovom diplomskom radu ispitan je utjecaj pohrane animalne rožnice u komercijalno dostupnim medijima za transport i pohranu rožnica na očuvanje barijernih svojstava rožnice. Barijerna svojstva rožnica procijenjena su određivanjem transepitelnog električnog otpora. Transepitelni električni otpor izoliranih animalnih rožnica određen je u vertikalnim protočnim difuzijskim ćelijama. Pohranom rožnica u CorneaPrepII® mediju tijekom 24 sata, barijerna svojstva rožnica ostaju očuvana. Pohranom rožnica u CorneaMax® mediju tijekom 120 sati, barijerna svojstva rožnica se mijenjaju u ovisnosti o vremenu; nakon 24 sata čuvanja u mediju barijerna svojstva rožnice nisu bila narušena, dok nakon 120 sati u mediju dolazi do narušavanja barijernih svojstava rožnice.Given that the corneal epithelium is the main barrier to the transfer of the active substances through the cornea, preservation of the epithelim is important to conduct ex vivo permeability tests. The aim of this diploma thesis was to examine the effect of the storage of animal cornea in commercially available mediums for transport and storage on the barrier properties of the cornea. Barrier properties of the cornea were estimated by measuring transepithelial electric resistance. Transepithelial electric resistance tests of the isolated animal corneas were performed in vertical difusion cells, using epithelial voltage clamp. By storing the corneas in the CorneaPrepII® meduim for 24 hours, the barrier properties of the corneas remain preserved. By storing the corneas in the CorneaMax® medium for 120 hours, the barrier properties change depending of the time of storage; after 24 hours of storage in the medium, the barrier properties of the corneas remain preserved, while after 120 hours of storage barrier properties were disrupted