The effect of high salt dietary intake on leukocytes' distribution and activation in female Sprague-Dawley rats

Cilj: Utvrditi izražajnost proteina HIF-1α kod akutne i intermitentne izloženosti hiperbaričnoj oksigenaciji (HBO₂) na modelu zdravih Sprague-Dawley štakora. Materijali i metode: Zdravi Sprague-Dawley štakori, starosti 9 – 11 tjedana, podijeljeni su u četiri skupine (KONTROLA, A-HBO₂, 24H-HBO₂ i 4D-HBO₂). Kontrolna skupina nije bila izložena djelovanju HBO₂. Akutnom djelovanju HBO₂ izložene su skupine A-HBO₂ (žrtvovani odmah nakon izlaganja) i 24H-HBO₂ (žrtvovani 24 h nakon izlaganja), dok je intermitentnom djelovanju HBO₂ bila izložena 4D-HBO₂ skupina štakora. Izražaj HIF-1α proteina odreĎen je na uzorcima torakalne aorte SD štakora uz pomoć Western blot metode. Rezultati: Izražaj HIF-1α proteina statistički je značajno povećan u 24H-HBO₂ i 4D-HBO₂ skupinama u usporedbi s kontrolnom skupinom. Zaključak: Akutno i intermitentno izlaganje hiperbaričnoj oksigenaciji potiče izražajnost HIF-1α proteinaAim: The aim of this study was to determine the expression of HIF-1α protein in acute and intermittent exposure to hyperbaric oxygenation (HBO₂) in healthy Sprague-Dawley rats. Materials and Methods: Healthy Sprague-Dawley rats, 9 - – 11 weeks old, were divided into four groups (CONTROL, A-HBO₂, 24H-HBO₂ i 4D-HBO₂). The control group was not exposed to HBO₂. A-HBO₂ (sacrificed immediately after the exposure) and 24H-HBO₂ (sacrificed 24 hours after the exposure) rats were exposed to the acute HBO₂, while 4D-HBO₂ rats were exposed to the intermittent HBO₂. The expression of HIF-1α protein was determined on thoracic aorta samples of SD rats by the Western blot method. Results: The expression of HIF-1α protein was statistically significantly increased in 24H-HBO₂ and 4D-HBO₂ groups in comparison to the control group. Conclusion: Acute and intermittent exposure to hyperbaric oxygenation induces HIF-1α protein expression