Differentiation of gastrointestinal stromal tumors by immunohistochemistry.

CILJ ISTRAŽIVANJA: Utvrditi broj dijagnosticiranih gastrointestinalnih stromalnih tumora u desetogodišnjem razdoblju, te učestalost prema biološkom ponašanju i lokalizaciji. USTROJ STUDIJE: Retrospektivna studija. MATERIJALI I METODE: Izvršila se analiza postojeće medicinske dokumentacije Kliničkog zavoda za patologiju i sudsku medicinu dijagnosticiranih gastrointestinalnih stromalnih tumora u KBC-u Osijek. Uzorci su bojani standardnim hematoksilin-eozin bojenjem i imunohistokemijskim bojenjem. Imunohistokemijski su određeni CD117, CD34, SMA i Ki-67. REZULTI: U desetogodišnjem razdoblju GIST je dijagnosticiran kod 57 pacijenata, a muškarci su bili više zastupljeni (57,89 %). Lokalizacija tumora najčešća je u želucu, a u ovoj studiji pojavlju se u debelom i tankom crijevu također. Prema NIH-u (engl. National Institute of Health – NIH) većina tumora bila je u području visokog rizika. Metastatski procesi uočeni su kod 16 pacijenata sa širenjem bolesti u regionalne limfne čvorove. Imunohistokemijski svi gastrointestinalni stromalni tumori pokazivali su izraženu pozitivnost na CD117. ZAKLJUČAK: Za dijagnostiku gastrointestinalnih stromalnih tumora neophodna je imunohistokemijska obrada tumorskog tkiva na specifične markere. U ovom istraživanju marker CD117 pokazao se izrazito specifičnim.OBJECTIVES: The aim of this study was to determine the number of gastrointestinal stromal tumours in a ten year period, to determine frequency depending on its biological behaviour and localization. STUDY DESIGN: Retrospective study. PARTICIPANTS AND METHODS: This study included existing medical record son diagnosed gastrointestinal stromal tumours at the Clinical Department of Pathology and Forensic Medicine at Clinical Hospital Centre Osijek. Samples were stained with standard hemalaun eosin method and immunohistochemistry staining. CD117, CD34, SMA and Ki - 67 were detected by immunohistochemistry methods. RESULTS: In the ten year period, 57 patients were diagnosed with GIST, most of whom were men (57.89 %). Localization of GIST is the most common on the stomach, but in this study, they also appear on the small and large intestine. According to National Institute of Health the most of tumours were in high risk area. Metastatic processes were present in 16 patients, most of them on regional lymph nodes. Immunohistochemistry indicated positivity on CD117 marker. CONSLUSION: For proper diagnostics of gastrointestinal stromal tumors immunohistochemistry is necessary in order to detect specific markers on the tumorous tissue. In this study marker CD117 is highly specific