Antifungal effect of garlic oil on species of Aspergillus genera

Eterična ulja su složene smjese različitih spojeva koji se dobivaju iz biljnog materijala različitim postupcima. Posjeduju antibakterijska, antifungalna, antivirusna, insekticidna i druga svojstva. Zbog svog antimikrobnog učinka, mogu se koristiti kao alternativan način konzerviranja namirnica, pri čemu im produžuju trajnost i doprinose senzorskim svojstvima iste. Zadatak rada bio je ispitati antifungalni učinak eteričnog ulja kineskog češnjaka (Allium tuberosum) i dialil – trisulfida na dvije vrste plijesni: Aspergillus flavus (soj 3.2758 i 3.4408) i A. oryzae. Najotpornija plijesan na djelovanje ulja češnjaka je soj A. flavus 3.4408 (minimalna fungicidna koncentracija ulja 1000 ppm), dok su soj A. flavus 3.2758 i A. oryzae inaktivirani pri 500 ppm. Dialil – trisulfid je za sve ispitane vrste djelovao fungicidno pri koncentraciji od 500 ppm. Germinacija spora plijesni i linearni rast kolonije su ovisne o primijenjenoj koncentraciji. Pri nižem aktivitetu vode (0,95) snažniji je antifungalni učinak ispitanog ulja češnjaka.Essential oils are complex mixtures of different compounds obtained from plants by different extraction procedures. Many different essential oils have antibacterial, antifungal, virucidal, insecticidal and other effects. In respect to their antimicrobial effect they can be used as an alternative preservatives in food industry, extending shelf life and sensory attributes of processed food. Main objective of this thesis was to asses antifungal effect of Chinese garlic (Allium tuberosum) essential oil and diallil – trisulfide on two species of Aspergillus genera: A. flavus (strains 3.2758 and 3.4408) and A. oryzae. The most resistant specie was A. flavus 3.4408 (minimal fungicidal concentration 1000 ppm) while A. flavus 3.2758 and A. oryzae were inactivated at 500 pp. All tested species were inactivated by diallil – trisulfide at 500 ppm. Spore germination and inhibition of linear colony growth are concentration dependent. At lower water activity applied (0,95) , antifungal effect of Chinese garlic is more pronounced