Assessment of breast cancer phenotype by immunohistochemistry.

CILJ ISTRAŽIVANJA. Utvrditi broj dijagnosticiranih karcinoma dojke u dvogodišnjem razdoblju, utvrditi učestalost karcinoma dojke prema fenotipu te usporediti zastupljenost pojedinih histoloških tipova u odnosu na fenotip karcinoma dojke. USTROJ STUDIJE. Retrospektivna poredbena studija. ISPITANICI I METODE. Studijom je obuhvaćeno 363 novo otkrivenih duktalnih in situ karcinoma dojke (DCIS) i invazivnih karcinoma dojke dijagnosticiranih na Zavodu za patologiju i sudsku medicinu KBC-a Osijek. Kod svih dijagnosticiranih karcinoma, imunohistokemijski su određeni estrogeni i progesteronski receptori, HER2 antigen i Ki67 proliferacijski indeks. Za imunohistokemijsku obradu tkiva koristio se automatski bojač Ventana Benchmark Ultra tvrtke Roche i monoklonalna primarna antitijela tvrtke Roche namijenjena za laboratorijsku uporabu. REZULTATI. U razdoblju od 1. siječnja 2015. godine do 31. prosinca 2016. godine na Zavodu za patologiju i sudsku medicinu KBC-a Osijek dijagnosticirano je 342 invazivnih karcinoma dojke. Najveći broj ispitanika bio je u dobnim skupinama od 60 do 69 godina (31,58 %), s prosječnom životnom dobi od 61,6 godina. Najzastupljeniji invazivni karcinomi dojke prema histološkome tipu su bili duktalni invazivni karcinomi (87,43 %), a prema fenotipu luminal B HER negativnoga fenotipa (48,54 %). Duktalni (48,16 %) i lobularni (52,94 %) invazivni karcinomi dojke te miješani (75 %) karcinomi bili su najčešće luminal B HER negativnoga tipa. Medularni karcinomi (75 %) većinom su bili trostruko negativnoga fenotipa, kao i mucinozni karcinom. ZAKLJUČAK. U dijagnostici invazivnih karcinoma dojke neophodna je imunohistokemijska obrada tkiva karcinoma radi određivanja točnoga fenotipa karcinoma s ciljem određivanja prikladne terapije.OBJECTIVES. To determine a number of diagnosed breast carcinomas in a two-year period. To determine a frequency of breast carcinomas according to a phenotype. To compare the presence of certain histological types with reference to the phenotype of breast carcinomas. STUDY DESIGN. A retrospective comparative study. PARTICIPANTS AND METHODS. The study includes 363 newly discovered ductal in situ breast carcinomas (DCIS) and invasive breast carcinomas diagnosed at the Clinical Institute for Pathology and Forensic Medicine at the Clinical Hospital Centre Osijek. By applying the method of immunohistochemistry, estrogen, progesterone, HER-2/neu and Ki-67 were determined. In order to immunohistochemically process tissues, the automatic counter Ventana BenchMark Ultra, produced by the company Roche, and monoclonal primal antibodies of the company Roche, made for the laboratory use, were used. RESULTS. In the period from January 2015 until December 2016, 342 invasive breast carcinomas were diagnosed at the Clinical Institute for Pathology and Forensic Medicine at the Clinical Hospital Centre Osijek. The majority of the examinees were at the age of 60 to 90 (31.58%) with the average age of 61.6 years. According to the histological type, the most frequent invasive breast carcinomas were ductal invasive carcinomas (87.43%). Furthermore, according to the phenotype, the most frequent was luminal B HER negative phenotype (48.54%). Ductal (48.16%) and lobular (52.94%) invasive breast carcinomas and mixed (75%) carcinomas were frequently luminal B of the negative type. Medullary carcinomas (75%) as well as mucinous carcinoma were mostly of the triple negative phenotype. CONCLUSION. In order to diagnose invasive breast carcinomas, the immunohistochemical treatment of a tissue is indispensable. It aims to determine the exact phenotype of carcinomas consequently determining the adequate therapy