Bioluminescence analysis and numerical evaluation of atp-synthesis by native and reconstituted membranes containing bacterial atp-synthase

The biological energy conservation resulting in ATP synthesis was observed by the luciferase bioluminescence test. The analysis was done as kinetic test with single samples taken in miute intervals. The non-linearity of the luciferase raw data was corrected with a computer program and a calibration curve estimated with each batch of analytical reagent containing luciferase and luciferin. The bioanalytical test was applied woth reconstituted liposomes containing ATP-synthase and monomeric bacteriorhodopsin, and with native bacterial plasma membrane vesicles (PMV) from Micrococcus luteus, which were energized by a NADH-O2 reaction