Analysis of a Metagenomic Sample Obtained by Third Generation Sequencing Technology

Brza i jeftina analiza metagenomskog uzorka korisna je za kontrolu kvalitete hrane, dijagnozu bolesti i utvrd̄ivanje štetnih nametnika na biljkama. Tradicionalne labora- torijske metode su ili dugotrajne ili namijenjene za samo jednu vrstu. Korištenjem ured̄aja treće generacije dobivaju se očitanja koja su puno dulja, no sadrže i znatno veći postotak pogrešaka. Implementiran je alat koji utvrd̄uje koji su organizmi prisutni u metagenomskim uzorcima dobivenenima koristeći upravo ured̄aje treće generacije. Alat u prvom koraku pronalazi sve organizme čiji genetski materijal nalikuje očita- njima iz uzorka, a zatim u drugom koraku analizira rezultate iz prvog koraka i utvrd̄uje koji su organizmi prisutni u zadanom uzorku.A fast and inexpensive analysis of metagenomic samples is useful for food quality control, medical diagnosis and identifying harmful plant parasites. Traditional labo- ratory methods are either time-consuming or designed for a specific species. Third- generation devices produce reads that are much longer, but also have a significantly higher error rate. A tool is implemented to determine which organisms are present in metagenomic samples obtained via third-generation devices. In its first step, the tool finds all organisms whose genetic material resembles that of the sample’s reads. Af- terwards, in the second step, the tool analyzes the first step’s results and determines which organisms are present in the given sample