Real-TimeAnalysis of a Metagenomic Sample Obtained by Nanopore Based Sequencing Technology

Brza i jeftina analiza metagenomoskog uzorka može biti korisna za dijagnostiku bolesti, kontrolu kvalitete hrane i utvrđivanje štetnih nametnika na biljkama. Tradicionalne laboratorijske metode su ili dugotrajne ili namijenjene za samo jednu vrstu. Uređaji za sekvenciranje MinION tvrtke Oxford Nanopore Technologies relativno su jeftini i pogodni za rad na terenu jer su lako prenosivi. Napravljen je alat koji u prvom koraku pronalazi sve organizme čije sekvence u svom dijelu imaju veliku sličnost s očitanim uzorcima, a nakon toga se utvrđuje koji organizmi su stvarno prisutni u tom uzorku.Fast and cheap metagenomic sample analysis could be very useful for medical diagnosis, food quality control and discovering harmful parasites on plants. Traditional laboratory methods are either long-lasting or oriented towards a single pathogen species. MinION sequencing devices by Oxford Nanopore Technologies are relatively cheap and suitable for application in the field. We developed a tool which in the first step discovers all organisms whose sequence have high similarity to the reads obtained from the samples and after that determines the organisms present in the sample