Determination of antioxidant and cholinesterase inhibitory activity of non-volatile isolates from comfrey root : diploma thesis

Oksidacijski stres definira se kao pomak ravnoteže u staničnim oksidacijsko-redukcijskim reakcijama u smjeru oksidacije. Riječ je o stanju prekomjernog stvaranja ili nedovoljno učinkovitog uklanjanja reaktivnih kisikovih vrsta. Tada dolazi do neuravnoteženog stvaranja slobodnih radikala i premašivanja mogućnosti neke stanice da ih razgradi, što rezultira oštećenjem stanica. Otkrivanje novih prirodnih antioksidansa i inhibitora enzima acetilkolinesteraze i butirilkolinesteraze moglo bi imati važnu ulogu u prevenciji štetnih oksidacijskih procesa i ublažavanju simptoma kod neurodegenerativnih oboljenja poput Alzheimerove i Parkinsonove bolesti. U ovom istraživanju korištena je biljka gavez (Symphytum officinale L.). Cilj ovog rada bio je odrediti sadržaj ukupnih fenola, ispitati antioksidacijska svojstava i sposobnost inhibicije enzima acetilkolinesteraze (AChE) i butirilkolinesteraze (BChE) vodenog, metanolnog i diklormetanskog ekstrakta biljke. Sadržaj ukupnih fenola određen je primjenom Folin-Ciocalteu metode, antioksidacijska aktivnost je ispitana metodama hvatanja slobodnih radikala (DPPH) i mjerenjem redukcijskog potencijala (FRAP), dok je sposobnost inhibicije kolinesterazaodređena metodom po Ellmanu. Vodeni ekstrakt je pokazao relativno dobra antioksidacijska svojstva. Uzorci nisu pokazali inhibicijski potencijal na kolinesterazeOxidation stress is defined as the shift of equilibrium in cellular oxidation-reduction reactions in the direction of oxidation. This is a state of excessive creation or inadequate removal of reactive oxygen species. Then there is an imbalance in the creation of free radicals and overcoming the ability of a cell to disrupt them, resulting in cell damage. Detection of new natural antioxidants and acetylcholinesterase and butyrylcholinesterase enzyme inhibitors could play an important role in the prevention of harmful oxidation processes and the alleviation of symptoms in neurodegenerative diseases such as Alzheimer's and Parkinson's disease. In this study, a comfrey plant (Symphytum officinale L.) was used. The aim of this paper was to determine the content of total phenols, to examine the antioxidant properties and the ability to inhibit the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes of the aqueous, methanolic and dichloromethane extract of the plant. The content of total phenols was determined using the Folin-Ciocalte method, antioxidant activity was tested by free radical capture methods (DPPH) and measurement of reduction potential (FRAP), while the ability of cholinesterase inhibition was determined by Ellman's method. The aqueous extract showed relatively good antioxidant properties. Samples did not show inhibitory potential on cholinesterase