QUALITATIVE ANALYSIS OF BABY CREAMS AND PREPARATIONS USING GC-MS METHOD

Ciljevi istraživanja: Kvalitativna analiza dječjih krema, usporedba dobivenih rezultata s deklaracijom proizvoda te pronalazak poveznice između cijene proizvoda i prisustva nepoželjnih sastojaka. Ustroj istraživanja: Eksperimentalna studija Mjesto istraživanja: Kemijsko-toksikološki laboratorij Kliničkog odjela za sudsku medicinu Zavoda za patologiju, sudsku medicinu i citologiju, KBC Split Materijali i metode: Ispitano je 10 uzoraka dječjih krema, raspoređenih u dva cjenovna ranga: 5 proizvoda s cijenom nižom i 5 s cijenom višom od 47,66 kn/100 mL. Za analitičku pripremu 0,1g uzorka ekstrahiran je smjesom otapala: kloroform, etilacetat i N-heksan (v/v/v = 1:1:1). Za usporedbu učinka otapala na ekstrakciju, uzorak br. 1 dodatno je otopljen u samom kloroformu. Kvalitativna analiza provedena je GC-MS metodom. Rezultati: Ispitivani nepoželjni sastojci su ftalati, parabeni, fenoksietanol, BHA i BHT, lanolin i parfemi. U 80% uzoraka detektiran je barem jedan od traženih nepoželjnih sastojaka dječjih krema, pri čemu u pet proizvoda koji pripadaju skupini nižeg te tri proizvoda koji pripadaju skupini višeg cjenovnog ranga. Odstupanje od deklaracije za detektirane nepoželjne sastojke zabilježeno je za dietilftalat i BHT, i to u skupini proizvoda nižeg cjenovnog ranga. Usporedbom kromatograma uzorka br. 1 pripremljenog na dva načina, prednost je dana smjesi otapala. Zaključak: Usporedbom cijene proizvoda u kojima su detektirani nepoželjni sastojci nisu dobiveni nedvojbeni rezultati. Utvrđena je povezanost cijene proizvoda i podudaranja detektiranih sastojaka s deklaracijom na ambalaži, odstupanja su prisutna samo u skupini nižeg cjenovnog ranga. Potrebna je provedba daljnjih ispitivanja u cilju detekcije iznimno niskih koncentracija nepoželjnih i nedeklariranih sastojaka.Objectives: Qualitative determination of ingredients in children skincare products, comparison of the results with the product declaration and finding a correlation between the price of the product and the presence of undesirable ingredients. Design: Experimental study Settings: Laboratory of toxicology, Department of pathology, medicine and cytology, University Hospital of Split Materials and methods: Ten samples of baby creams were analyzed and divided into two price ranges: 5 products with price lower and 5 products with price greater than 47.66 kn/100 mL. 0.1g of each sample was extracted with a mixture of solvents: chloroform, ethylacetate and N-hexane (v/v/v=1:1:1). In order to compare the results of different solvents, sample no.1 was dissolved in chloroform as well. Qualitative analysis was preformed by GC-MS method. Results: Undesirable ingredients investigated in this study are phthalates, parabens, phenoxyethanol, BHA and BHT, lanolin and perfume. In 80% of samples at least one of the tested ingredients was detected, with 5 products belonging to the lower and 3 products belonging to the higher price range group. The deviation from the declaration for these detected undesirable substances was recorded for diethylphthalate and BHT, only in the lower price range product group. By comparing the chromatograms of sample no.1 prepared in two ways, a mixture of solvents was preferred. Conclusion: Correlation between price of the product and presence of undesirable ingredients was not conclusive. Correlation between the price of the product and deviations from the product declaration is established, with deviations present only in the lower price range group. More thorough studies are required to detect extremely low concentrations of undesirable and undeclared ingredients