Heavy metal determination by biosensors based on enzyme immobilised by electropolymerisation.
- MALITESTA, Cosimino
- GUASCITO, Maria Rachele
Publication date
January 2005
DOI Publisher
Elsevier BV ISSN
0956-5663 Citation count (estimate)
68Abstract
The work describes the original application of biosensors based on enzyme immobilised by electropolymerisation to heavy metal determination. An inhibition detection scheme has been employed for detecting Hg2+ by an established glucose biosensor based on glucose oxidase immobilised in poly-o-phenylenediamine. The investigated enzymatic inhibition appears reversible and mixed, in agreement with data for the enzyme in solution. A low response time (<2 min) and a rapid recovery of response by EDTA seem the most interesting characteristics of the proposed biosensor at the present stage of development, along with the well known easy preparation of this kind of biosensors. The occurrence of a high response also for Cu2+ opens the possibility ...
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