Human exposure to Bisphenol A and liver health status: Quantification of urinary and circulating levels by LC-MS/MS

A selective and highly sensitive analytical methodology for determination of Bisphenol A in human plasma was developed and validated. The method was based on selective liquid/solid extraction, combined with liquid chromatography-electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode and negative ionization. The linearity of the detector response was verified in human plasma over the concentration range 0.100-200ngmL(-1). The detection limit was 0.03ngmL(-1) and the quantification limit was 0.100ngmL(-1). The analytical features of the proposed in-house validated method were satisfactory: precision was