Cytotoxicity of Two Bioactive Root Canal Sealers

Uvod: Svrha istraživanja bila je ispitati citotoksičnost dvaju različitih bioaktivnih materijala za punjenje korijenskih kanala temeljenih na mineral-trioksidnom agregatu MTA Fillapex (Angelus, Solucoes Odontologicas, Londrina, PR, Brazil) i biokeramici, Endosequence BC Sealer (Brasseler, Savannah, Georgia, SAD) u kulturi mišjih fibroblasta L929. Materijali i postupci: Mišji fibroblasti L929, dobiveni iz potkožnog veziva miševa linije C3Hf, uzgojeni su u plastičnim posudama za staničnu kulturu površine 75 cm2 u inkubatoru na temperaturi od 37 ºC, uz 5-posto CO2 i 90 posto vlažnosti. Svježe zamiješani materijali – (0,1 g) Endosequence BC Sealer i MTA Fillapex – naneseni su na sterilne teflonske diskove promjera šest milimetara. Diskovi s materijalom i prazni teflonski diskovi koji su služili kao kontrola, stavljeni su u bunariće pločica za staničnu kulturu. Nakon inkubacije od jedan sat, šest, 20 i 24 sata, uklonjeni su teflonski diskovi i određen je broj živih stanica tripanskim modrilom u Neubaerovoj komorici. Rezultati: Promatranjem razlike između ispitivanih materijala i kontrolne skupine u pojedinim inkubacijskim razdobljima, dokazano je da punilo MTA u svim inkubacijskim razdobljima pokazuje statistički značajan pad broja živih stanica (p ≤ 0,05), a kod punila BC pojavljuje se statistički značajna razlika od šestog do dvadeset i četvrtog sata inkubacije (p ≤ 0,05). Punilo MTA u odnosu na punilo BC pokazalo je statistički značajan pad broja živih stanica samo nakon prvog i dvadesetog sata inkubacije (p ≤ 0,05), a u ostalim inkubacijskim razdobljima ta razlika nije bila statistički značajna (p ≥ 0,05). Zaključak: Punila MTA i Endosequence BC bila su citotoksična u kulturi mišjih fibroblasta.Objective: The aim of this study was to investigate the cytotoxicity of two different bioactive root canal sealers: one based on mineral trioxide aggregate, MTA Fillapex (Angelus, Solucoes Odontologicas, Londrina, PR, Brazil), and the other based on bioceramics, Endosequence BC Sealer (Brasseler, Savannah, Georgia, USA), in culture of mouse L929 fibroblasts. Materials and methods: Mouse fibroblasts (L929), obtained from subcutaneous connective tissue of mouse line C3Hf, were cultivated in plastic culture flasks in an incubator at 37ºC, with 5% CO2 and 90% humidity. Freshly mixed Endosequence BC Sealer and MTA Fillapex (0.1 g each) were placed on sterile teflon discs, 6 mm in diameter. Teflon discs with the materials as well as empty discs serving as control were placed in wells of 12-well plate. After incubation times of 1, 6, 20 and 24 hours, the teflon discs were removed from the wells and the number of viable cells was determined using trypan blue in Neubauer chamber. Results: In comparison to the control group, MTA Fillapex had significantly less viable cells for all incubation periods (p≤0.05), while Endosequence BC sealer had significantly less viable cells after 6, 20, and 24 hours of incubation (p≤0.05). MTA Fillapex exhibited ignificantly less viable cells in comparison to Endosequence BC sealer after the first hour and after 20 hours of incubation (p≤0.05), while for the other incubation periods there were no significant differences (p≥0.05). Conclusion: MTA Fillapex and Endosequence BC sealer were both cytotoxic in cultures of mouse L929 fibroblasts