Preimplantation genetic diagnosis for the treatment of failed in vitro fertilization-embryo transfer and habitual abortion.

Objective: To apply preimplantation genetic diagnosis (PGD) for the treatment of patients with a history of failed FVF-ET or habitual aborters. Design: Prospective clinical study. Setting: Tertiary center for assisted reproduction. Patient(s): Ninety-four couples with failed IVF-ET after >2 F/F cycles and 64 couples with >2 spontaneous abortions. Intervention(s): Patients were prepared for oocyte retrieval using standard controlled ovarian hyperstimulation protocols after standard laboratory techniques. Blastomeres from 6- to 8-cell embryos were analysed using fluorescence in situ hybridization with commercial chromosomal probes, and normoploid embryos were transferred on day 3 after fertilization. Main Outcome Measure(s): Pregnancy and implantation rates and live births. Result(s): Both 3- and 5-probe PGD resulted in a significantly higher outcome than controls for failed IVF-ET. Five-probe PGD appeared to be more suitable for habitual aborters. Conclusion(s): This pilot study suggests that 3-probe PGD is a valid option for failed IVF-ET patients. The use of five or more probes is indicated for habitual aborters