ACTIVATION OF INNATE AND NOT ADAPTIVE IMMUNE SYSTEM IN GLUTEN SENSITIVITY

Background and aim: There are cases of gluten reaction defined as gluten sensitivity (GS) in which neither an allergic (wheat allergy) noran autoimmune [celiac disease (CD)] mechanism can be advocated. Recent evidences suggest that early changes in intestinal permeability (IP) and activation of both innate and adaptive immune responses are involved in CD pathogenesis. Conversely, no data are currently available on the mechanisms leading to GS. Aim was to investigate the changes in IP, TJ protein genes expression, and innate and adaptive immune responses in GS. Material and methods: Biopsy samples were obtained from 28 GS patients, 53 pts with active CD, 16 patients with CD in remission, and 37 healthy controls (age range: 5 years -50 years). Claudin (CL) 1, CL2, CL3, CL4, ZO-1, and TLR1, TLR2 and TLR4, FOXP3, and TGF-β gene expression were performed by Real-time PCR. IP was evaluated by means of the lactulose/mannitol test (LA/MA). The number of intraepithelial lymphocytes (IELs) was detected with CD3 and TCR-γδ immunostaining and examined by counting the peroxidase stained cells. ELISA analysis of IL6, IL8, TNFα, was conducted on PBMC of all patients. Results: CL3 and CL4 expressions were significantly increased in GS subjects compared to CD patients (p<0.01). In GS patients, these changes were associated to a lower IP (0.010±0.008) that inversely correlated to CL4 gene expression (r= -0.6318; p