Determination of lysosomal membrane stability in nussels Mytilus galloprovincialis Lamarck, 1819. from the Northern Adriatic

Stabilnost lizosomalnih membrana u probavnoj žlijezdi dagnji koristi se kao pokazatelj onečišćenja okoliša. U ovom radu je istražen učinak teških metala (kadmij, olovo i živa) i trofičkog indeksa na stabilnost lizosomalnih membrana u probavnoj žlijezdi dagnji Mytilus galloprovincialis uzorkovanih u kolovozu 2016. godine na postajama Limski kanal, Rovinj, Pula, Brestova, Rijeka i Bakar. Osim toga, u ovom radu istražen je i stupanj onečišćenja za svih šest postaja u sjevernom Jadranu, gdje postaje Pula, Rijeka i Bakar pokazuju najveće onečišćenje. Stabilnost lizosomalnih membrana određena je histokemijskom metodom po Moore-u, a podaci o koncentracijama metala u mekom tkivu školjkaša i trofički indeks istraženi su iz literature. Dagnje na svim postajama pokazuju stresne uvjete, ali najniže vrijednosti stabilnosti lizosomalnih membrana utvrđene su u dagnjama uzrokovanim na postajama Bakar i Lim. Rezultati ovog istraživanja pokazuju da promjene ekoloških čimbenika ponekad mogu maskirati utjecaj onečišćenja, stoga je potrebno koristiti više parametara pri procjeni stanja morskog okoliša.Lysosomal membrane stability in mussel digestive gland is widely used biomarker that respond to pollutant exposure. In this paper, we studied the effect of heavy metals (cadmium, lead and mercury) and the trophic index on the lysosomal membrane stability in the digestive gland of mussels Mytilus galloprovincialis. Mussels were sampled in August 2016 at the stations Lim Channel, Rovinj, Pula, Brestov, Rijeka and Bakar, Northern Adriatic, Croatia. In addition, in this paper the pollution level for all six stations in the northern Adriatic was investigated. Stations, Pula, Rijeka and Bakar were showed the highest level of pollution. The lysosomal membrane stability was determined by the histochemical method by Moore, and metal concentration in mussel tissue and trofic index was investigated from the literature. Mussels sampled at all exhibit stressful conditions, but the lowest values of the lysosomal membrane stability are found in the mussels sampled at the Bakar and Lim. The results of this research showed that changes in ecological factors might mask the effects of pollution, and more parameters should be included in assessing the quality of the marine environment