The origin analysis of croatian Barbary sheep (Ammotragus lervia PALLAS,1777) population using mitochondrial DNA sequences

Grivasti skakač (Ammotragus lervia Pallas, 1777) životinjska je vrsta kojoj je izvorno stanište usko geografsko područje Sjeverne Afrike. Pripada porodici Bovidae i brojnost u Republici Hrvatskoj se procjenjuje na 200 jedinki. S obzirom da je objavljeno vrlo malo znanstvenih podataka o hrvatskoj populaciji grivastog skakača, cilj ovog diplomskog rada bio je analizirati raznolikost kontrolne regije mitohondrijske DNA grivastog skakača na području Hrvatske i usporediti dobivene sekvence sa sekvencama iz NCBI baze sekvenci. Istraživanje je provedeno na uzorcima prikupljenim s područja Mosora i analiziran je slijed kontrolne regije duljine 1075 parova baza. Pronađeno je jedno polimorfno mjesto koje čini dva haplotipa. Haplotipska raznolikost analiziranih slijedova iznosila je 0,530, dok je nukleotidna raznolikost iznosila 0,00050. Na temelju dobivenih rezultata zaključujem da je genetička raznolikost kontrolne regije mitohondrijske DNA grivastog skakača iz Hrvatske relativno niska.The barbary sheep (Ammotragus lervia Pallas, 1777) is an animal species originally inhabited by the narrow geographic region of North Africa. It belongs to the family Bovidae and the population in Croatia is estimated at 200 individuals. Considering that a very little scientific data on Croatian population was published, the aim of this graduate thesis was to analyze the diversity of the control region of the mitochondrial DNA in barbary sheep in Croatia and to compare identified sequences with sequences from NCBI base of sequences. The study was carried out on samples collected from the Mosor area and a sequence of control regions 1075 base pairs long was analysed. One polymorphic site was found which makes two haplotypes. The haplotype diversity of the analyzed sequences was 0.530, while the nucleotide diversity was 0.00050. Based on these results, I can conclude that the genetic diversity of the control region of the mitochondrial DNA of the barbary sheep from Croatia is relatively low