© 2016, Springer Science+Business Media New York.Nitroxide spin labelling is exploited in electron paramagnetic resonance (EPR) spectroscopy to study biological interactions and analysis of conformational changes in protein structures. In our study, recombinant human histone H1.3 was modified by 3-carboxy-proxyl using carbodiimide/N-hydroxysuccinimide coupling. Resulting conjugate of histone H1.3 with the spin label preserved its DNA binding capacity and formed complexes with plasmid DNA. The changes in EPR signal of the spin label were revealed upon conjugate interaction with plasmid DNA. Our preliminary data show the possibility of DNA sensing with nitroxide spin labelled histone H1.3 by EPR spectroscopy
2018-07-15Clustered, regularly-interspaced, palindromic repeats (CRISPR) systems derived from bacter...
Publisher's version (útgefin grein)Pulsed electron paramagnetic resonance (EPR) experiments, among t...
Publisher's version (útgefin grein)Three 2´-deoxynucleosides containing semi-flexible spin labels, n...
© 2016, Springer Science+Business Media New York.Nitroxide spin labelling is exploited in electron p...
Post-print (lokagerð höfundar)Electron paramagnetic resonance (EPR) spectroscopy, coupled with site-...
AbstractNitroxide labels are useful probes of biomolecule structure that can be detected by NMR and ...
Nitroxide labels are useful probes of biomolecule structure that can be detected by NMR and EPR spec...
Typescript.Thesis (M. S.)--University of Hawaii at Manoa, 1975.Bibliography: leaves [143]-152.xv, 15...
Histone octamer tails play an important role in regulating transcription factor binding and nucleoso...
Precise and temporal regulation of DNA-templated processes is maintained by the hierarchical systems...
The (H3-H4)(2) histone tetramer forms the central core of nucleosomes and, as such, plays a prominen...
Molecular modeling has proven to be a powerful tool for studying structure and dynamics of biologica...
A variety of semirigid and rigid spin labels comprise a valuable arsenal for measurements of biomole...
UnrestrictedIn site-directed spin labeling, a covalently attached nitroxide probe containing a chemi...
Eukaryotic DNA is packaged into chromatin to fit in the limited dimensions of the nucleus. The basic...
2018-07-15Clustered, regularly-interspaced, palindromic repeats (CRISPR) systems derived from bacter...
Publisher's version (útgefin grein)Pulsed electron paramagnetic resonance (EPR) experiments, among t...
Publisher's version (útgefin grein)Three 2´-deoxynucleosides containing semi-flexible spin labels, n...
© 2016, Springer Science+Business Media New York.Nitroxide spin labelling is exploited in electron p...
Post-print (lokagerð höfundar)Electron paramagnetic resonance (EPR) spectroscopy, coupled with site-...
AbstractNitroxide labels are useful probes of biomolecule structure that can be detected by NMR and ...
Nitroxide labels are useful probes of biomolecule structure that can be detected by NMR and EPR spec...
Typescript.Thesis (M. S.)--University of Hawaii at Manoa, 1975.Bibliography: leaves [143]-152.xv, 15...
Histone octamer tails play an important role in regulating transcription factor binding and nucleoso...
Precise and temporal regulation of DNA-templated processes is maintained by the hierarchical systems...
The (H3-H4)(2) histone tetramer forms the central core of nucleosomes and, as such, plays a prominen...
Molecular modeling has proven to be a powerful tool for studying structure and dynamics of biologica...
A variety of semirigid and rigid spin labels comprise a valuable arsenal for measurements of biomole...
UnrestrictedIn site-directed spin labeling, a covalently attached nitroxide probe containing a chemi...
Eukaryotic DNA is packaged into chromatin to fit in the limited dimensions of the nucleus. The basic...
2018-07-15Clustered, regularly-interspaced, palindromic repeats (CRISPR) systems derived from bacter...
Publisher's version (útgefin grein)Pulsed electron paramagnetic resonance (EPR) experiments, among t...
Publisher's version (útgefin grein)Three 2´-deoxynucleosides containing semi-flexible spin labels, n...