Isoforms of Serratia marcescens nuclease. The role of Mg2+ in the hydrolysis mechanism

  • Filimonova M.
  • Gubskaya V.
  • Nuretdinov I.
  • Benedik M.
  • Bogomolnaya L.
  • Andreeva M.
  • Leshchinskaya I.
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Publication date
January 1997
Journal
Biochemistry (Moscow)

Abstract

Structural and functional differences between isoforms Sm1 and Sm2, a lack of influence of free Mg2+ on the isoform structures, formation of DNA-magnesium complex serving with great probability as a real substrate for the nuclcasc has been summarized on the basis of experimental data. Mg2+ forming a complex with phosphate groups of DNA are supposed to further increase the electrophilicity of the phosphorus atoms besides causing a conformational change of the substrate

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