Histochemical Demonstration of Lipids in Cultured Porcine Oocytes and Preimplantation Embryos

Takano, Hiroko
Niimura, Sueo

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Publication date

January 2004

DOI

10.1274/jmor.21.36

Publisher

Japanese Society of Mammalian Ova Research

ISSN

1341-7738

Journal

Journal of Mammalian Ova Research

Citation count (estimate)

Abstract

Changes in the number of lipid droplets during meiotic maturation, fertilization and early development were histochemically examined in cultured porcine oocytes and embryos. The oocytes and embryos possessed Sudanophilic lipids composed of small (<2.5 μm), medium (2.5-4.9 μm) and large (≥5.0 μm) droplets. In oocytes soon after collection, the numbers of Sudanophilic lipid droplets with small and medium sizes were few and the number of those with large size was 148 ± 11.36. After being cultured for 22 and 44 hrs, the number of lipid droplets with large size remarkably decreased, while the number of those with small and medium sizes increased. The numbers of lipid droplets of each size in the oocytes 4 and 8 hrs after insemination were simila...