Cold-stress in Escherichia coli induces de novo synthesis of translation initiation factors IF1, IF2 and IF3 while ribosome synthesis and assembly slow down. Consequently, the IFs/ribosome stoichiometric ratio increases about 3-fold during the first hours of cold adaptation. The IF1 and IF3 increase plays a role in translation regulation at low temperature (cold-shock-induced translational bias) but so far no specific role could be attributed to the extra copies of IF2. In this work, we show that the extra-copies of IF2 made after cold stress are associated with immature ribosomal subunits together with at least another nine proteins involved in assembly and/or maturation of ribosomal subunits. This finding, coupled with evidence that IF2 i...
Substitution of the conserved Histidine 448 present in one of the three consensus elements character...
Translation initiation factor 1 (IF1) has been shown to be an RNA chaperone. In order to find functi...
The cold shock response in both Escherichia coli and Bacillus subtilis is induced by an abrupt downs...
Cold-stress in Escherichia coli induces de novo synthesis of translation initiation factors IF1, IF2...
After a 37 to 10°C temperature downshift the level of translation initiation factor IF2, like that o...
Lolita Piersimoni, Mara Giangrossi, Claudio O. Gualerzi and Cynthia L. Pon Laboratory of Genetics,...
Bacterial translation initiation factor IF1 is an S1 domain protein that belongs to the oligomer bin...
Upon temperature downshift below the lower threshold of balanced growth (∼20°C), the Escherichia col...
Research carried out in the last two decades has shown that all living organisms, from bacteria to m...
To ensure the correct assembly of ribosomal subunits, ribosomal RNA (rRNA)and ribosomal protein (r-p...
Translation initiation factor IF2 is a guanine nucleotide-binding protein. The free energy change as...
Lolita Piersimoni, Mara Giangrossi, Claudio O. Gualerzi & Cynthia L. Pon\ud \ud Laboratory of Geneti...
There is a considerable interest in the cold adaptation of food-related bacteria, including starter ...
There is a considerable interest in the cold adaptation of food-related bacteria, including starter ...
Temperature influences the structural and functional properties of cellular components, necessitatin...
Substitution of the conserved Histidine 448 present in one of the three consensus elements character...
Translation initiation factor 1 (IF1) has been shown to be an RNA chaperone. In order to find functi...
The cold shock response in both Escherichia coli and Bacillus subtilis is induced by an abrupt downs...
Cold-stress in Escherichia coli induces de novo synthesis of translation initiation factors IF1, IF2...
After a 37 to 10°C temperature downshift the level of translation initiation factor IF2, like that o...
Lolita Piersimoni, Mara Giangrossi, Claudio O. Gualerzi and Cynthia L. Pon Laboratory of Genetics,...
Bacterial translation initiation factor IF1 is an S1 domain protein that belongs to the oligomer bin...
Upon temperature downshift below the lower threshold of balanced growth (∼20°C), the Escherichia col...
Research carried out in the last two decades has shown that all living organisms, from bacteria to m...
To ensure the correct assembly of ribosomal subunits, ribosomal RNA (rRNA)and ribosomal protein (r-p...
Translation initiation factor IF2 is a guanine nucleotide-binding protein. The free energy change as...
Lolita Piersimoni, Mara Giangrossi, Claudio O. Gualerzi & Cynthia L. Pon\ud \ud Laboratory of Geneti...
There is a considerable interest in the cold adaptation of food-related bacteria, including starter ...
There is a considerable interest in the cold adaptation of food-related bacteria, including starter ...
Temperature influences the structural and functional properties of cellular components, necessitatin...
Substitution of the conserved Histidine 448 present in one of the three consensus elements character...
Translation initiation factor 1 (IF1) has been shown to be an RNA chaperone. In order to find functi...
The cold shock response in both Escherichia coli and Bacillus subtilis is induced by an abrupt downs...