Sucrose and corn fiber hydrolysis using a succinic acid catalyst

There are a lot of the methods of hydrolysis at present. The choice of the method is dependent on the species and quality of the raw material, economical and ecological effectiveness of the technology and requirements of the final product. The hydrolysis of sucrose by succinic acid was investigated in a preliminary study. It was found that optimal conditions of sucrose hydrolysis correspond to 100ºC, a hydrolysis time of 1 hour and 0.5% concentration of succinic acid. The media, which consists of glucose / fructose approximately in ratio 50 : 50 was checked by a fermentation process with the E.coli mutant AFP 184. The fermentation resulted in a final succinic acid concentration of about 28.3 g/l after 16 hours of production. The regimes of the hydrolysis of corn fibre biomass (0.125mm particles) by succinic acid were also examined. The influences of temperature, time and succinic acid concentration on the hydrolysis were studied. It was found that the most favourable conditions for hydrolysis are 200ºC and 5% succinic acid concentration. Two types of corn fibre pretreatment were analyzed. The results demonstrate the difference of preboiling at 100ºC or 160ºC for 30 min. Pretreatment at 160ºC results in a higher degree of sugar degradation than pretreatment at 100ºC. The analysis of the hydrolysate showed that the total concentration of monosaccharides was 20g/l.Validerat; 20101217 (root